Journal: Aging (Albany NY)
Article Title: The tumor microenvironment in gastrointestinal adenocarcinomas revealed a prognostic and immunotherapeutic biomarker
doi: 10.18632/aging.204463
Figure Lengend Snippet: TMEscore was an independent factor for predicting patient survival . ( A ) The identification flowchart of six prognostic genes (C6orf223, EPHX4, HES6, NKD2 OLR1 and ONECUT2). ( B ) The expression of the six genes in GIAC tumor and normal samples from TCGA cohort. ( C ) The RNA expression of the six genes in 55 pairs of CRC tumor and normal samples from our center (GAD and ESAD see in , ). ( D ) The expression of the five proteins in 10 pairs of CRC tumor and normal samples from our center (GAD and ESAD see in , ). ( E ) The WB image of protein expression for CRC (GAD and ESAD see in , ). ( F – K ) The survival time (OS and PFS) of patients with high/low TMEscore in TCGA cohort ( F , G ), GSE17536 ( H , I ) and GSE39582 ( J , K ).
Article Snippet: Primary antibodies used in this study include rabbit anti-β-tubulin (Abcam, ab6046, 1:1000 dilution for WB), rabbit anti-EPHX4 (Abcam, ab183739, 1:1000 dilution for WB), mouse anti-HES6 (Abcam, ab172800, 1:1000 dilution for WB), rabbit anti-NKD2 (CST, 2073T, 1:1000 dilution for WB), rabbit anti-OLR1 (Bioss, bs-2044R, 1:1000 dilution for WB) and anti-ONECUT2 (Bioss, bs-19643R, 1:1000 dilution for WB).
Techniques: Expressing, RNA Expression